Ultrasonication Extraction and Solid Phase Extraction Clean-up for Quantification of Aristolochic Acid I in the Thai Herbal Antitussive 3 Formula by High Performance Liquid Chromatography with Photodiode Array Detection
Objective: To establish a reliable High Performance Liquid Chromatography method with photodiode array detection (HPLC-PDA) and a suitable extraction method for identification and quantification of Aristolochic acid I (AA-I) in 3 different sample types, i.e.Aristolochia tagala Cham.(ATC), Thai Herbal Antitussive 3 Formula (ATF3), and Thai Herbal An